Bacteria Counts with On-Chip Flow Cytometry on the Agilent 2100 Bioanalyzer System
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چکیده
This Application Note describes an alternative process for bacterial cell counting using on-chip fl ow cytometry with the Agilent 2100 Bioanalyzer system. This procedure is described with three different kinds of prokaryotes: Escherichia coli (gram-negative, rod-shaped), Pseudomonas aeruginosa (gram-negative, rod-shaped), and Staphylococcus aureus (gram-positive, coccal). As a reference for bacterial concentration, optical density of the bacterial suspension was measured, as well as obtaining hemocytometer counts. Bacteria Counts with On-Chip Flow Cytometry on the Agilent 2100 Bioanalyzer System
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